Article
Estimating mutant microsatellite allele frequencies in somatic cells by small-pool PCR.
Genomics - 1 Aug 2004
Coolbaugh-Murphy Mary, Maleki Atousa, Ramagli Lou, Frazier Marsha, Lichtiger Benjamin, Monckton Darren G, Siciliano Michael J, Brown Barry W
Abstract excerpt
Identifying microsatellite instability (MSI) by partitioning DNA into multiple small pools containing only single genome amounts of DNA results in trapping both progenitor and low-frequency mutant alleles into pools where they can be identified and counted following PCR. Statistical approaches determining both the frequencies and the significant differences between frequencies of these Poisson-distributed alleles...
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