Article
Role of the G protein-coupled receptor kinase site serine cluster in beta2-adrenergic receptor internalization, desensitization, and beta-arrestin translocation.
The Journal of biological chemistry - 17 Mar 2006
Vaughan David J, Millman Ellen E, Godines Veronica, Friedman Jacqueline, Tran Tuan M, Dai Wenping, Knoll Brian J, Clark Richard B, Moore Robert H
Abstract excerpt
There is considerable evidence for the role of carboxyl-terminal serines 355, 356, and 364 in G protein-coupled receptor kinase (GRK)-mediated phosphorylation and desensitization of beta(2)-adrenergic receptors (beta(2)ARs). In this study we used receptors in which these serines were changed to alanines (SA3) or to aspartic acids (SD3) to determine the role of these sites in beta-arrestin-dependent beta(2)AR...
Topics
- Adenylyl Cyclases
- Alanine
- Arrestins
- Aspartic Acid
- Binding Sites
- Blotting, Western
- Cell Line
- Cell Membrane
- Cyclic AMP-Dependent Protein Kinases
- DNA, Complementary