Article
The nuclease domain of the Escherichia coli RecBCD enzyme catalyzes degradation of linear and circular single-stranded and double-stranded DNA.
Biochemistry - 10 Jan 2006
Sun Jian-Zhong, Julin Douglas A, Hu Jin-Shan
Abstract excerpt
The 30 kDa C-terminal domain of the RecB protein (RecB30) has nuclease activity and is believed to be responsible for the nucleolytic activities of the RecBCD enzyme. However, the RecB30 protein, studied as a histidine-tagged fusion protein, appeared to have very low nucleolytic activity on single-stranded (ss) DNA [Zhang, X. J., and Julin, D. A. (1999) Nucleic Acids Res. 27, 4200-4207], which raised the question...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Catalytic Domain
- Cations, Divalent
- DNA
- DNA, Single-Stranded
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli Proteins
- Exodeoxyribonuclease V
- Metals
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Nucleic Acid Conformation
- Time Factors