Article
Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.
The Journal of biological chemistry - 4 Nov 2005
Spies Maria, Dillingham Mark S, Kowalczykowski Stephen C
Abstract excerpt
RecBCD enzyme is a heterotrimeric helicase/nuclease that initiates homologous recombination at double-stranded DNA breaks. The enzyme is driven by two motor subunits, RecB and RecD, translocating on opposite single-strands of the DNA duplex. Here we provide evidence that, although both motor subunits can support the translocation activity for the enzyme, the activity of the RecB subunit is necessary for proper...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Binding Sites
- DNA Helicases
- DNA, Bacterial
- DNA, Single-Stranded
- Escherichia coli Proteins
- Exodeoxyribonuclease V
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic