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Article

Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity.

Journal of molecular biology - 5 Jun 1993

Eggleston A K, Kowalczykowski S C

Abstract excerpt

RecBCD enzyme of Escherichia coli is a DNA helicase which also possesses ATP-dependent nuclease activities. We have purified a mutant recBCD enzyme, designated recB2109CD enzyme, and have examined the nuclease activities of this protein in vitro to determine whether any alteration in these activities is responsible for the recombination-deficient phenotype of the recB2109 strain. The recB2109CD enzyme possesses...

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Identifiers

PMID: 8390577
DOI: 10.1006/jmbi.1993.1313

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity.PMID 8390577Read ↗

The mutant recBCD enzyme, recB2109CD enzyme, has helicase activity but does not promote efficient joint molecule formation in vitro.Semantic proximity Read ↗

Genetic dissection of the biochemical activities of RecBCD enzyme.Semantic proximity Read ↗

The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.Semantic proximity Read ↗

Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme.Semantic proximity Read ↗

Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.Semantic proximity Read ↗

Nuclease activity is essential for RecBCD recombination in Escherichia coli.Semantic proximity Read ↗

The nuclease domain of the Escherichia coli RecBCD enzyme catalyzes degradation of linear and circular single-stranded and double-stranded DNA.Semantic proximity Read ↗

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.Semantic proximity Read ↗

Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli.Semantic proximity Read ↗

RecBCD enzyme overproduction impairs DNA repair and homologous recombination in Escherichia coli.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

Purification and biochemical characterization of Escherichia coli RecA proteins mutated in the putative DNA binding site.Semantic proximity Read ↗

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