Evidence that recBC-dependent degradation of duplex DNA in Escherichia coli recD mutants involves DNA unwinding.

doi:10.1128/jb.174.16.5424-5429.1992

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Article

Evidence that recBC-dependent degradation of duplex DNA in Escherichia coli recD mutants involves DNA unwinding.

Journal of bacteriology - 1 Aug 1992

Rinken R, Thomas B, Wackernagel W

Abstract excerpt

Infection of Escherichia coli with phage T4 gene 2am was used to transport 3H-labeled linear duplex DNA into cells to follow its degradation in relation to the cellular genotype. In wild-type cells, 49% of the DNA was made acid soluble within 60 min; in recB or recC cells, only about 5% of the DN...

Topics

DNA Helicases
DNA, Bacterial
DNA, Viral
Escherichia coli
Escherichia coli Proteins
Exodeoxyribonuclease V
Exodeoxyribonucleases
Mutation
Phenotype
T-Phages
Thymidine

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