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Article

The mutant recBCD enzyme, recB2109CD enzyme, has helicase activity but does not promote efficient joint molecule formation in vitro.

Journal of molecular biology - 5 Jun 1993

Eggleston A K, Kowalczykowski S C

Abstract excerpt

The Escherichia coli recB2109CD enzyme displays a defect in homologous recombination. In vitro, it possesses significant levels of non-specific nuclease activity but is deficient in chi-dependent nicking activity. To determine whether an alteration in helicase activity contributes further to its in vivo defect, the ability of recB2109CD enzyme to unwind dsDNA was examined. The mutant enzyme is able to unwind DNA...

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PMID: 8390578
DOI: 10.1006/jmbi.1993.1314

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SSB

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The mutant recBCD enzyme, recB2109CD enzyme, has helicase activity but does not promote efficient joint molecule formation in vitro.PMID 8390578Read ↗

Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity.Semantic proximity Read ↗

Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme.Semantic proximity Read ↗

The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.Semantic proximity Read ↗

Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.Semantic proximity Read ↗

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.Semantic proximity Read ↗

Genetic dissection of the biochemical activities of RecBCD enzyme.Semantic proximity Read ↗

A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in vivo.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

Nuclease activity is essential for RecBCD recombination in Escherichia coli.Semantic proximity Read ↗

RecBCD enzyme overproduction impairs DNA repair and homologous recombination in Escherichia coli.Semantic proximity Read ↗

Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli.Semantic proximity Read ↗

Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.Semantic proximity Read ↗

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