The mutant recBCD enzyme, recB2109CD enzyme, has helicase activity but does not promote efficient joint molecule formation in vitro.

doi:10.1006/jmbi.1993.1314

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Article

The mutant recBCD enzyme, recB2109CD enzyme, has helicase activity but does not promote efficient joint molecule formation in vitro.

Journal of molecular biology - 5 Jun 1993

Eggleston A K, Kowalczykowski S C

Abstract excerpt

The Escherichia coli recB2109CD enzyme displays a defect in homologous recombination. In vitro, it possesses significant levels of non-specific nuclease activity but is deficient in chi-dependent nicking activity. To determine whether an alteration in helicase activity contributes further to its...

Topics

Adenosine Triphosphate
DNA
DNA Helicases
Escherichia coli
Exodeoxyribonuclease V
Exodeoxyribonucleases
Kinetics
Magnesium
Mutation
Sodium Chloride

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