Article
Genetic dissection of the biochemical activities of RecBCD enzyme.
Genetics - 1 Sept 1990
Amundsen S K, Neiman A M, Thibodeaux S M, Smith G R
Abstract excerpt
RecBCD enzyme of Escherichia coli is required for the major pathway of homologous recombination following conjugation. The enzyme has an ATP-dependent DNA unwinding activity, ATP-dependent single-stranded (ss) and double-stranded (ds) DNA exonuclease activities, and an activity that makes a ss DNA endonucleolytic cut near Chi sites. We have isolated and characterized ten mutations that reduced recombination...
Topics
- Adenosine Triphosphate
- Coliphages
- DNA Helicases
- DNA Transposable Elements
- Drug Resistance, Microbial
- Escherichia coli
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Genetic Complementation Test
- Mitomycin
- Mitomycins