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Article

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.

The Journal of biological chemistry - 17 Sept 1999

Anderson D G, Churchill J J, Kowalczykowski S C

Abstract excerpt

Homologous recombination and double-stranded DNA break repair in Escherichia coli are initiated by the multifunctional RecBCD enzyme. After binding to a double-stranded DNA end, the RecBCD enzyme unwinds and degrades the DNA processively. This processing is regulated by the recombination hot spot, Chi (chi: 5'-GCTGGTGG-3'), which induces a switch in the polarity of DNA degradation and activates RecBCD enzyme to...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 10480929
DOI: 10.1074/jbc.274.38.27139

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.PMID 10480929Read ↗

Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.Semantic proximity Read ↗

A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in vivo.Semantic proximity Read ↗

Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme.Semantic proximity Read ↗

Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.Semantic proximity Read ↗

A domain of RecC required for assembly of the regulatory RecD subunit into the Escherichia coli RecBCD holoenzyme.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.Semantic proximity Read ↗

Chi and the RecBC D enzyme of Escherichia coli.Semantic proximity Read ↗

The mutant recBCD enzyme, recB2109CD enzyme, has helicase activity but does not promote efficient joint molecule formation in vitro.Semantic proximity Read ↗

Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli.Semantic proximity Read ↗

Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity.Semantic proximity Read ↗

Role of the Escherichia coli recombination hotspot, chi, in RecABCD-dependent homologous pairing.Semantic proximity Read ↗

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