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Article

Isolation and characterization of the C-terminal nuclease domain from the RecB protein of Escherichia coli.

Nucleic acids research - 1 Nov 1999

Zhang X J, Julin D A

Abstract excerpt

The RecB subunit of the Escherichia coli RecBCD enzyme has been shown in previous work to have two domains: an N-terminal 100 kDa domain with ATP-dependent helicase activity, and a C-terminal 30 kDa domain. The 30 kDa domain had nuclease activity when linked to a heterologous DNA binding protein, but by itself it appeared unable to bind DNA and lacked detectable nuclease activity. We have expressed and isolated...

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Identifiers

PMID: 10518611
DOI: 10.1093/nar/27.21.4200
PMCID: PMC148694

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Isolation and characterization of the C-terminal nuclease domain from the RecB protein of Escherichia coli.PMID 10518611Read ↗

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