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Article

Nuclease activity is essential for RecBCD recombination in Escherichia coli.

Molecular microbiology - 1 Aug 2001

Jockovich M E, Myers R S

Abstract excerpt

RecBCD has two conflicting roles in Escherichia coli. (i) As ExoV, it is a potent double-stranded (ds)DNA exonuclease that destroys linear DNA produced by restriction of foreign DNA. (ii) As a recombinase, it promotes repair of dsDNA breaks and genetic recombination in the vicinity of chi recombination hot-spots. These paradoxical roles are accommodated by chi-dependent attenuation of RecBCD exonuclease activity...

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Identifiers

PMID: 11532156
DOI: 10.1046/j.1365-2958.2001.02573.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Nuclease activity is essential for RecBCD recombination in Escherichia coli.PMID 11532156Read ↗

Genetic dissection of the biochemical activities of RecBCD enzyme.Semantic proximity Read ↗

Chi and the RecBC D enzyme of Escherichia coli.Semantic proximity Read ↗

Chi hotspot activity in Escherichia coli without RecBCD exonuclease activity: implications for the mechanism of recombination.Semantic proximity Read ↗

The recombination hot spot chi activates RecBCD recombination by converting Escherichia coli to a recD mutant phenocopy.Semantic proximity Read ↗

The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.Semantic proximity Read ↗

RecBCD enzyme overproduction impairs DNA repair and homologous recombination in Escherichia coli.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.Semantic proximity Read ↗

Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme.Semantic proximity Read ↗

Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity.Semantic proximity Read ↗

Recombinational repair of chromosomal DNA double-strand breaks generated by a restriction endonuclease.Semantic proximity Read ↗

Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.Semantic proximity Read ↗

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