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Article

Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme.

The Journal of biological chemistry - 21 Apr 2000

Arnold D A, Kowalczykowski S C

Abstract excerpt

Although the RecB(2109)CD enzyme retains most of the biochemical functions associated with the wild-type RecBCD enzyme, it is completely defective for genetic recombination. Here, we demonstrate that the mutant enzyme exhibits an aberrant double-stranded DNA exonuclease activity, intrinsically producing a 3'-terminal single-stranded DNA overhang that is an ideal substrate for RecA protein-promoted strand...

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Source

Identifiers

PMID: 10766864
DOI: 10.1074/jbc.275.16.12261

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Click a neighbor to travelStep 1 · 12 closest

Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme.PMID 10766864Read ↗

Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.Semantic proximity Read ↗

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.Semantic proximity Read ↗

The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.Semantic proximity Read ↗

Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity.Semantic proximity Read ↗

A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in vivo.Semantic proximity Read ↗

The mutant recBCD enzyme, recB2109CD enzyme, has helicase activity but does not promote efficient joint molecule formation in vitro.Semantic proximity Read ↗

Nuclease activity is essential for RecBCD recombination in Escherichia coli.Semantic proximity Read ↗

Genetic dissection of the biochemical activities of RecBCD enzyme.Semantic proximity Read ↗

Chi and the RecBC D enzyme of Escherichia coli.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

RecBCD enzyme overproduction impairs DNA repair and homologous recombination in Escherichia coli.Semantic proximity Read ↗

Role of the Escherichia coli recombination hotspot, chi, in RecABCD-dependent homologous pairing.Semantic proximity Read ↗

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