Crude isolation of DNA from unselected human pancreatic tissue and amplification by the polymerase chain reaction of Ki-ras oncogene to detect point mutations in pancreatic cancer.

Abstract excerpt

Human pancreatic tissue obtained during surgery was divided into two samples, one stored in formalin for histology and the other frozen directly for DNA studies. A small bit of the frozen tissue was excised without prior differentiation of malignant from non-malignant tissue and crude DNA prepared. The DNA was used to amplify the Ki-ras oncogenes with the polymerase chain reaction (PCR) using primers for codons...

Topics

• Codon
• DNA Mutational Analysis
• DNA, Neoplasm
• Genes, ras
• Humans
• Mutation
• Nucleic Acid Amplification Techniques
• Pancreas
• Pancreatic Neoplasms
• Polymerase Chain Reaction