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Article

Analysis of transmembrane domain mutants is consistent with sequential cleavage of Notch by gamma-secretase.

Journal of neurochemistry - 1 Jan 2006

Chandu Dilip, Huppert Stacey S, Kopan Raphael

Abstract excerpt

gamma-Secretase is a lipid-embedded, intramembrane-cleaving aspartyl protease that cleaves its substrates twice within their transmembrane domains (TMD): once near the cytosolic leaflet (at S3/epsilon) and again in the middle of the TMD (at S4/gamma). To address whether this unusual process occurs in two independent or interdependent steps, we investigated how mutations at the S3/epsilon site in Notch1-based...

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Identifiers

PMID: 16300632
DOI: 10.1111/j.1471-4159.2005.03547.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Analysis of transmembrane domain mutants is consistent with sequential cleavage of Notch by gamma-secretase.PMID 16300632Read ↗

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Permissive Conformations of a Transmembrane Helix Allow Intramembrane Proteolysis by γ-Secretase.Semantic proximity Read ↗

Mutation analysis of the presenilin 1 N-terminal domain reveals a broad spectrum of gamma-secretase activity toward amyloid precursor protein and other substrates.Semantic proximity Read ↗

Mutations at the P1' position of Notch1 decrease intracellular domain stability rather than cleavage by gamma-secretase.Semantic proximity Read ↗

Three-amino acid spacing of presenilin endoproteolysis suggests a general stepwise cleavage of gamma-secretase-mediated intramembrane proteolysis.Semantic proximity Read ↗

Size-dependent protein segregation creates a spatial switch for Notch signaling and functionSemantic proximity Read ↗

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Proteolytic Processing of Neuregulin 1 Type III by Three Intramembrane-cleaving Proteases.Semantic proximity Read ↗

Presenilin-1 D257A and D385A mutants fail to cleave Notch in their endoproteolyzed forms, but only presenilin-1 D385A mutant can restore its gamma-secretase activity with the compensatory overexpression of normal C-terminal fragment.Semantic proximity Read ↗

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