Article
Enhanced inhibition of polymerization of sickle cell hemoglobin in the presence of recombinant mutants of human fetal hemoglobin with substitutions at position 43 in the gamma-chain.
Biochemistry - 13 Sept 2005
Tam Ming F, Chen Jun, Tam Tsuey-Chyi S, Tsai Ching-Hsuan, Shen Tong-Jian, Simplaceanu Virgil, Feinstein Timothy N, Barrick Doug, Ho Chien
Abstract excerpt
Four recombinant mutants of human fetal hemoglobin [Hb F (alpha2gamma2)] with amino acid substitutions at the position 43 of the gamma-chain, rHb (gammaD43L), rHb (gammaD43E), rHb (gammaD43W), and rHb (gammaD43R), have been expressed in our Escherichia coli expression system and used to investigate their inhibitory effect on the polymerization of deoxygenated sickle cell hemoglobin (Hb S). Oxygen-binding studies...
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