Roles of alpha 114 and beta 87 amino acid residues in the polymerization of hemoglobin S: implications for gene therapy.

doi:10.1006/jmbi.1996.0590

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Roles of alpha 114 and beta 87 amino acid residues in the polymerization of hemoglobin S: implications for gene therapy.

Journal of molecular biology - 1 Nov 1996

Ho C, Willis B F, Shen T J, Dazhen N T, Sun D P, Tam M F, Suzuka S M, Fabry M E, Nagel R L

Abstract excerpt

Three novel recombinant mutants of sickle hemoglobin (Hb S, beta 6Glu-->Val) have been constructed to assess the role of proline at alpha 114 and threonine at beta 87 in the polymerization of deoxygenated Hb S. Using the hemoglobin expression system (pHE2) designed in our laboratory, four plasmids were expressed separately in Escherichia coli to produce the four recombinant hemoglobins: r Hb S (beta 6Glu-->Val);...

Topics

Adult
Anemia, Sickle Cell
Escherichia coli
Fetal Hemoglobin
Genetic Therapy
Hemoglobin A
Hemoglobin, Sickle
Humans
Magnetic Resonance Spectroscopy
Mutation
Oxygen

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymers

Proline

Protein Conformation

Recombinant Proteins

Sequence Analysis

Solubility

Threonine

Source

Open in PubMed

Identifiers

PMID: 8918602
DOI: 10.1006/jmbi.1996.0590

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8918602-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Roles of alpha 114 and beta 87 amino acid residues in the polymerization of hemoglobin S: implications for gene therapy.PMID 8918602Read ↗

Enhanced inhibition of polymerization of sickle cell hemoglobin in the presence of recombinant mutants of human fetal hemoglobin with substitutions at position 43 in the gamma-chain.Semantic proximity Read ↗

Recombinant human hemoglobins designed for gene therapy of sickle cell disease.Semantic proximity Read ↗

Recombinant sickle hemoglobin containing a lysine substitution at Asp-85(alpha): expression in yeast, functional properties, and participation in gel formation.Semantic proximity Read ↗

Role of Leu-beta 88 in the hydrophobic acceptor pocket for Val-beta 6 during hemoglobin S polymerization.Semantic proximity Read ↗

Targeting sickle cell disease root-cause pathophysiology with small moleculesSemantic proximity Read ↗

Role of beta87 Thr in the beta6 Val acceptor site during deoxy Hb S polymerization.Semantic proximity Read ↗

Properties of a recombinant human hemoglobin double mutant: sickle hemoglobin with Leu-88(beta) at the primary aggregation site substituted by Ala.Semantic proximity Read ↗

Impact of hemoglobin biophysical studies on molecular pathogenesis and drug therapy for sickle cell diseaseSemantic proximity Read ↗

Sickle hemoglobin polymer stability probed by triple and quadruple mutant hybrids.Semantic proximity Read ↗

A Recombinant Human Hemoglobin with Anti-sickling Properties Greater than Fetal HemoglobinSemantic proximity Read ↗

Sickle Cell Anemia: Targeting the Role of Fetal Hemoglobin in TherapySemantic proximity Read ↗

Haemoglobin alpha 2 beta 2 23Val----Ile produced in Escherichia coli facilitates Hb S polymerization.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.