Article
Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo.
Nucleic acids research - 1 Jan 2005
Holen Torgeir, Moe Svein Erik, Sørbø Jan Gunnar, Meza Trine J, Ottersen Ole Petter, Klungland Arne
Abstract excerpt
RNA interference (RNAi) has become an invaluable tool for functional genomics. A critical use of this tool depends on an understanding of the factors that determine the specificity and activity of the active agent, small interfering RNA (siRNA). Several studies have concluded that tolerance of mutations can be considerable and hence lead to off-target effects. In this study, we have investigated in vivo the...
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