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Article

Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs.

Nucleic acids research - 1 Dec 2009

Huang Huang, Qiao Renping, Zhao Deyao, Zhang Tong, Li Youxian, Yi Fan, Lai Fangfang, Hong Junmei, Ding Xianfeng, Yang Zhenjun, Zhang Lihe, Du Quan, Liang Zicai

Abstract excerpt

Silencing specificity is a critical issue in the therapeutic applications of siRNA, particularly in the treatment of single nucleotide polymorphism (SNP) diseases where discrimination against single nucleotide variation is demanded. However, no generally applicable guidelines are available for the design of such allele-specific siRNAs. In this paper, the issue was approached by using a reporter-based assay. With...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 19815667
DOI: 10.1093/nar/gkp835
PMCID: PMC2794185

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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