Article
Screening mutant libraries of fungal laccases in the presence of organic solvents.
Journal of biomolecular screening - 1 Sept 2005
Alcalde Miguel, Bulter Thomas, Zumárraga Miren, García-Arellano Humberto, Mencía Mario, Plou Francisco J, Ballesteros Antonio
Abstract excerpt
Reliable screening methods are being demanded by biocatalysts' engineers, especially when some features such as activity or stability are targets to improve under non-natural conditions (i.e., in the presence of organic solvents). The current work describes a protocol for the design of a fungal laccase-expressed in Saccharomyces cerevisiae-highly active in organic cosolvents. A high-throughput screening assay...
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