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Re-engineering of <i>CUP1</i> promoter and Cup2/Ace1 transactivator to convert <i>Saccharomyces cerevisiae</i> into a whole-cell eukaryotic biosensor capable of detecting 10 nM of bioavailable copper

2022-04-04

Abstract excerpt

While copper is an essential micronutrient and a technologically indispensable heavy metal, it is toxic at high concentrations, harming the environment and human health. Currently, copper is monitored with costly and low-throughput analytical techniques that do not evaluate bioavailability, a crucial parameter which can be measured only with living cells. We overcame these limitations by building upon yeast S. ce...

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Literature Corpus work
5e447704-3fed-51b8-add9-f7e6dcca90c7
DOI
10.1101/2022.04.04.486947
Open publication

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Re-engineering of <i>CUP1</i> promoter and Cup2/Ace1 transactivator to convert <i>Saccharomyces cerevisiae</i> into a whole-cell eukaryotic biosensor capable of detecting 10 nM of bioavailable copperDOI 10.1101/2022.04.04.486947
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