Article
Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes.
Biotechnology journal - 1 Apr 2009
Brissos Vânia, Pereira Luciana, Munteanu Florentina-Daniela, Cavaco-Paulo Artur, Martins Lígia O
Abstract excerpt
Laccases are useful biocatalysts for many diverse biotechnological applications. In this study we have established efficient and reliable expression systems and high-throughput screenings for the recombinant CotA-laccase from Bacillus subtilis. The expression levels of cotA-laccase were compared in five different Escherichia coli host strains growing in 96-well microtiter plates under different culture...
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