Article
Engineering CotA Laccase for Acidic pH Stability Using Bacillus subtilis Spore Display.
Journal of microbiology and biotechnology - 28 Mar 2017
Sheng Silu, Jia Han, Topiol Sidney, Farinas Edgardo T
Abstract excerpt
Bacillus subtilis spores can be used for protein display to engineer protein properties. This method overcomes viability and protein-folding concerns associated with traditional protein display methods. Spores remain viable under extreme conditions and the genotype/phenotype connection remains intact. In addition, the natural sporulation process eliminates protein-folding concerns that are coupled to the target...
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