Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae.

Scientific reports - 24 Feb 2020

Kwiatos Natalia, Jędrzejczak-Krzepkowska Marzena, Krzemińska Agnieszka, Delavari Azar, Paneth Piotr, Bielecki Stanisław

Abstract excerpt

Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards 2,6-dimethoxyphenol, that could be used as a mediator for lignin modification. A combination of classical culture optimization and protein engineering led to around 30 times higher activity in the culture supernatant. The winner mutant exhibited three...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Fusarium
Share to Kinetics
Share to Laccase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Molecular Docking Simulation

Share to Molecular Dynamics Simulation

Share to Protein Engineering

Share to Saccharomyces cerevisiae

Share to Thermodynamics

Source

Open in PubMed Open full text

Identifiers

PMID: 32094483
DOI: 10.1038/s41598-020-60204-1
PMCID: PMC7039978

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae.PMID 32094483Read ↗

In vitro evolution of a fungal laccase in high concentrations of organic cosolvents.Semantic proximity Read ↗

Screening mutant libraries of fungal laccases in the presence of organic solvents.Semantic proximity Read ↗

Protein Engineering Approaches to Enhance Fungal Laccase Production in S. cerevisiae.Semantic proximity Read ↗

Fungal cytochrome P450 monooxygenases of Fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered Saccharomyces cerevisiae.Semantic proximity Read ↗

Colorimetric High-Throughput Screening Assays for the Directed Evolution of Fungal Laccases.Semantic proximity Read ↗

Replacement of oxidizable residues predicted by QM-MM simulation of a fungal laccase generates variants with higher operational stability.Semantic proximity Read ↗

Directed evolution of glucose oxidase from Aspergillus niger for ferrocenemethanol-mediated electron transfer.Semantic proximity Read ↗

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast.Semantic proximity Read ↗

Expression in yeast of secreted lignin peroxidase with improved 2,4-dichlorophenol degradability by DNA shuffling.Semantic proximity Read ↗

Improvement of the fungal enzyme pyranose 2-oxidase using protein engineering.Semantic proximity Read ↗

Engineering platforms for directed evolution of Laccase from Pycnoporus cinnabarinus.Semantic proximity Read ↗

Improving the decolorization for textile dyes of a metagenome-derived alkaline laccase by directed evolution.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.