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Article

Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5'ss base pairing in yeast.

Molecular cell - 1 Jul 2005

Lacadie Scott A, Rosbash Michael

Abstract excerpt

To investigate the mechanism of spliceosome assembly in vivo, we performed chromatin immunoprecipitation (ChIP) analysis of U1, U2, and U5 small nuclear ribonucleoprotein particles (snRNPs) to intron-containing yeast (S. cerevisiae) genes. The snRNPs display patterns that indicate a cotranscriptional assembly model: U1 first, then U2, and the U4/U6*U5 tri-snRNP followed by U1 destabilization. cis-splicing...

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Identifiers

PMID: 15989965
DOI: 10.1016/j.molcel.2005.05.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5'ss base pairing in yeast.PMID 15989965Read ↗

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A U1-U2 snRNP interaction network during intron definition.Semantic proximity Read ↗

Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing.Semantic proximity Read ↗

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The conserved central domain of yeast U6 snRNA: importance of U2-U6 helix Ia in spliceosome assembly.Semantic proximity Read ↗

Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavage.Semantic proximity Read ↗

Multiple functions for the invariant AGC triad of U6 snRNA.Semantic proximity Read ↗

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