Article
Mutations in PurBox1 of the Bacillus subtilis pur operon control site affect adenine-regulated expression in vivo.
Science in China. Series C, Life sciences - 1 Apr 2005
Xuan Jinsong, Zalkin Howard, Weng Manli
Abstract excerpt
Transcription of the Bacillus subtilis pur operon is regulated by a purine repressor (PurR)-DNA control site interaction. The pur operon control site has two PurBoxes that are required for high-affinity PurR binding. An upstream, strong-binding PurBox1 is at position -81 to -68 relative to the transcription start site and a downstream weak-binding PurBox2 is at position -49 to -36. We constructed three PurBox1...
Topics
- Adenine
- Bacillus subtilis
- Bacterial Proteins
- Gene Expression Regulation, Bacterial
- Mutation
- Operator Regions, Genetic
- Operon
- Repressor Proteins
- beta-Galactosidase