Article
Mutational analysis of the Bacillus subtilis purA operator site.
Current microbiology - 1 Nov 2005
Rappu Pekka, Leppihalme Mari, Mäntsälä Pekka
Abstract excerpt
The Bacillus subtilis purine repressor, PurR, regulates many genes involved in purine metabolism. These genes contain a conserved 14-nucleotide inverted repeat (PurBox). Both pur operon and purA, which are regulated by PurR, have this inverted repeat with a 16- or 17-nucleotide spacer, respectively. Mutational studies have earlier shown that PurR binding is dependent on the PurBox of pur operon. In contrast,...
Topics
- Adenylosuccinate Synthase
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- Binding Sites
- DNA Mutational Analysis
- Gene Expression Regulation, Bacterial
- Genes, Reporter
- Luciferases
- Molecular Sequence Data
- Mutation