Article
In vivo effect of mutations at the PRPP binding site of the Bacillus subtilis purine repressor.
Journal of bacteriology - 1 Nov 2003
Rappu Pekka, Pullinen Terhi, Mäntsälä Pekka
Abstract excerpt
The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR.
Topics
- Bacillus subtilis
- Bacterial Proteins
- Binding Sites
- Gene Expression Regulation, Bacterial
- Mutation
- Phosphoribosyl Pyrophosphate
- Repressor Proteins