Article
Validation of recombinant and bovine chymosin by mass spectrometry.
Journal of agricultural and food chemistry - 29 Jun 2005
Lilla Sergio, Mogna Giovanni, Addeo Francesco
Abstract excerpt
Mass spectrometry has been used to map chymosin from a fermentative source. The copresence of the two known genetic variants A (Asp(244)) and B (Gly(244)) was ascertained in bovine chymosin. By contrast, either the A or the B genetic variant occurred in the three commercial samples of recombinant calf chymosin (RCC). Specific biomarker proteins were searched to identify the enzyme source, in both bovine chymosin...
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