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Article

Disruption of PMR1 in Kluyveromyces lactis improves secretion of calf prochymosin.

Journal of the science of food and agriculture - 15 Jan 2011

Feng Zhen, Ren Jing, Zhang Huajiang, Zhang Lanwei

Abstract excerpt

BACKGROUND: Chymosin is an important industrial enzyme widely used in cheese manufacturing. Kluyveromyces lactis is a promising host strain for expression of the chymosin gene. However, only low yields of chymosin (80 U mL(-1) in shake flask culture) have been obtained using K. lactis GG799. The aim of this study was to increase the amount of recombinant calf chymosin secreted by K. lactis GG799 by disrupting the...

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PMID: 20812383
DOI: 10.1002/jsfa.4156

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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