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Article

Precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases.

Protein engineering - 1 Oct 2003

Kühnel Blanka, Alcantara Joenel, Boothe Joseph, van Rooijen Gijs, Moloney Maurice

Abstract excerpt

Expression of recombinant proteins as translational fusions is commonly employed to enhance stability, increase solubility and facilitate purification of the desired protein. In general, such fusion proteins must be cleaved to release the mature protein in its native form. The usefulness of the procedure depends on the efficiency and precision of cleavage and its cost per unit activity. We report here the...

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Identifiers

PMID: 14600208
DOI: 10.1093/protein/gzg091

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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