Direct sequence data from heterogeneous creatine kinase (43 kDa) by high-resolution tandem mass spectrometry.

doi:10.1021/bi00050a004

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Article

Direct sequence data from heterogeneous creatine kinase (43 kDa) by high-resolution tandem mass spectrometry.

Biochemistry - 19 Dec 1995

Wood T D, Chen L H, Kelleher N L, Little D P, Kenyon G L, McLafferty F W

Abstract excerpt

Isoelectric focusing separation of recombinant rabbit muscle creatine kinase (CK) and its 282Cys-->282Ser mutant shows the presence of three and two isoforms, respectively, that exhibit equivalent enzymatic activity. Electrospray ionization coupled with Fourier-transform mass spectrometry (10(5)...

Topics

Animals
Creatine Kinase
Fourier Analysis
Isoenzymes
Mass Spectrometry
Molecular Weight
Muscles
Mutation
Protein Processing, Post-Translational
Rabbits
Sequence Analysis

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