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Article

Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli.

European journal of biochemistry - 1 Sept 1998

Nardone E, Rosano C, Santambrogio P, Curnis F, Corti A, Magni F, Siccardi A G, Paganelli G, Losso R, Apreda B, Bolognesi M, Sidoli A, Arosio P

Abstract excerpt

The mature hen avidin encoded by a synthetic cDNA was expressed in Escherichia coli in an insoluble form. After resolubilization, renaturation and purification, a recovery of about 20 mg/l cell culture was obtained. ELISA assays indicated no apparent differences in biotin binding between the natural and recombinant avidins. In addition, an acidic avidin mutant, bearing the substitutions Lys3-->Glu, Lys9--> Glu,...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 9760187
DOI: 10.1046/j.1432-1327.1998.2560453.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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