Article
Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli.
European journal of biochemistry - 1 Sept 1998
Nardone E, Rosano C, Santambrogio P, Curnis F, Corti A, Magni F, Siccardi A G, Paganelli G, Losso R, Apreda B, Bolognesi M, Sidoli A, Arosio P
Abstract excerpt
The mature hen avidin encoded by a synthetic cDNA was expressed in Escherichia coli in an insoluble form. After resolubilization, renaturation and purification, a recovery of about 20 mg/l cell culture was obtained. ELISA assays indicated no apparent differences in biotin binding between the natu...
Topics
- Animals
- Antibodies
- Antigens
- Avidin
- Binding, Competitive
- Biotin
- Biotinylation
- Chickens
- Crystallography, X-Ray
- Escherichia coli
- Gene Expression
- Isoelectric Point
- Mass Spectrometry
- Models, Molecular