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Article

Recombinant bovine rhodanese: purification and comparison with bovine liver rhodanese.

Biochimica et biophysica acta - 24 Jun 1992

Miller D M, Kurzban G P, Mendoza J A, Chirgwin J M, Hardies S C, Horowitz P M

Abstract excerpt

Recombinant bovine rhodanese (thiosulfate: cyanide sulfurtransferase, EC 2.8.1.1) has been purified to homogeneity from Escherichia coli BL21(DE3) by cation-exchange chromatography. Recombinant and bovine liver rhodanese coelectrophorese under denaturing conditions, with an apparent subunit molecular weight of 33,000. The amino terminal seven residues of the recombinant protein are identical to those of the...

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PMID: 1627606
DOI: 10.1016/0167-4838(92)90158-a

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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