Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutations in a highly conserved region of the Arf1p activator GEA2 block anterograde Golgi transport but not COPI recruitment to membranes.

Molecular biology of the cell - 1 Aug 2005

Park Sei-Kyoung, Hartnell Lisa M, Jackson Catherine L

Abstract excerpt

We have identified an important functional region of the yeast Arf1 activator Gea2p upstream of the catalytic Sec7 domain and characterized a set of temperature-sensitive (ts) mutants with amino acid substitutions in this region. These gea2-ts mutants block or slow transport of proteins traversing the secretory pathway at exit from the endoplasmic reticulum (ER) and the early Golgi, and accumulate both ER and...

Topics

ADP-Ribosylation Factor 1
Amino Acid Sequence
Biological Transport
Cell Membrane
Coat Protein Complex I
Genetic Vectors
Golgi Apparatus
Guanine Nucleotide Exchange Factors
Microscopy, Electron, Transmission
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Sequence Alignment

Source

Open in PubMed Open full text

Identifiers

PMID: 15930122
DOI: 10.1091/mbc.e05-04-0289
PMCID: PMC1182316

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-15930122-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutations in a highly conserved region of the Arf1p activator GEA2 block anterograde Golgi transport but not COPI recruitment to membranes.PMID 15930122Read ↗

The ARF exchange factors Gea1p and Gea2p regulate Golgi structure and function in yeast.Semantic proximity Read ↗

Sec7p directs the transitions required for yeast Golgi biogenesis.Semantic proximity Read ↗

Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi.Semantic proximity Read ↗

Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function.Semantic proximity Read ↗

COPI-independent anterograde transport: cargo-selective ER to Golgi protein transport in yeast COPI mutants.Semantic proximity Read ↗

ARF is required for maintenance of yeast Golgi and endosome structure and function.Semantic proximity Read ↗

Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function.Semantic proximity Read ↗

ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation.Semantic proximity Read ↗

The clathrin adaptor Gga2p is a phosphatidylinositol 4-phosphate effector at the Golgi exit.Semantic proximity Read ↗

ADP-ribosylation factor (ARF) interaction is not sufficient for yeast GGA protein function or localization.Semantic proximity Read ↗

Shared and specific functions of Arfs 1–5 at the Golgi revealed by systematic knockoutsSemantic proximity Read ↗

COPI vesicles accumulating in the presence of a GTP restricted arf1 mutant are depleted of anterograde and retrograde cargo.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.