Article
In resting COS1 cells a dominant negative approach shows that specific, anchored PDE4 cAMP phosphodiesterase isoforms gate the activation, by basal cyclic AMP production, of AKAP-tethered protein kinase A type II located in the centrosomal region.
Cellular signalling - 1 Sept 2005
McCahill Angela, McSorley Theresa, Huston Elaine, Hill Elaine V, Lynch Martin J, Gall Irene, Keryer Guy, Lygren Birgitte, Tasken Kjetil, van Heeke Gino, Houslay Miles D
Abstract excerpt
We employ a novel, dominant negative approach to identify a key role for certain tethered cyclic AMP specific phosphodiesterase-4 (PDE4) isoforms in regulating cyclic AMP dependent protein kinase A (PKA) sub-populations in resting COS1 cells. A fraction of PKA is clearly active in resting COS1 cells and this activity increases when cells are treated with the selective PDE4 inhibitor, rolipram. Point mutation of a...
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