Article
Sumoylation induced by the Arf tumor suppressor: a p53-independent function.
Proceedings of the National Academy of Sciences of the United States of America - 24 May 2005
Tago Kenji, Chiocca Susanna, Sherr Charles J
Abstract excerpt
The mouse p19(Arf) protein has both p53-dependent and p53-independent tumor-suppressive activities. Arf triggers sumoylation of many cellular proteins, including Mdm2 and nucleophosmin (NPM/B23), with which p19(Arf) physically interacts in vivo, and this occurs equally well in cells expressing or lacking functional p53. In an Arf-null NIH 3T3 cell derivative (MT-Arf cells) engineered to reexpress an Arf transgene...
Topics
- Animals
- Cell Line
- Cell Nucleolus
- Cyclin-Dependent Kinase Inhibitor p16
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Fluorescent Antibody Technique
- Genetic Vectors
- Humans
- Immunoblotting
- Mice