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Article

Affinity maturation of a V(H)H by mutational hotspot randomization.

Journal of immunological methods - 1 Feb 2005

Yau Kerrm Y F, Dubuc Ginette, Li Shenghua, Hirama Tomoko, Mackenzie C Roger, Jermutus Lutz, Hall J Christopher, Tanha Jamshid

Abstract excerpt

V(H)Hs from naive libraries have dissociation constants (K(D)s) in the low micromolar range and thus, for most antibody applications, their intrinsic affinities need to be improved significantly. Non-targeted in vitro affinity maturation approaches based on indiscriminate randomization of complementarity-determining region (CDR) residues or random mutagenesis of conventional antibody variable domains have been...

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Share to Antibody Affinity
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 15777944
DOI: 10.1016/j.jim.2004.12.005

Genes

CDR2
PTH

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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