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Article

Affinity maturation of antibodies assisted by in silico modeling.

Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 2008

Barderas Rodrigo, Desmet Johan, Timmerman Peter, Meloen Rob, Casal J Ignacio

Abstract excerpt

Rational engineering methods can be applied with reasonable success to optimize physicochemical characteristics of proteins, in particular, antibodies. Here, we describe a combined CDR3 walking randomization and rational design-based approach to enhance the affinity of the human anti-gastrin TA4 scFv. The application of this methodology to TA4 scFv, displaying only a weak overall affinity for gastrin17 (K(D) = 6...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 18574150
DOI: 10.1073/pnas.0801221105
PMCID: PMC2449359

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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