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Article

"Breeding" diagnostic antibodies for higher assay performance: a 250-fold affinity-matured antibody mutant targeting a small biomarker.

Analytical chemistry - 21 May 2013

Oyama Hiroyuki, Yamaguchi Shuko, Nakata Shigetoshi, Niwa Toshifumi, Kobayashi Norihiro

Abstract excerpt

Conventional immunization-based antibodies are not always applicable to monitor trace amounts of clinical biomarkers due to insufficient antigen-binding affinity. The development of in vitro affinity maturation for native antibodies has paved the way to solve this problem, but there has been minimal success in obtaining improved antibody mutants for small molecule biomarkers (haptens). We here describe a...

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PMID: 23631394
DOI: 10.1021/ac3037602

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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