Article
Two-step in vitro antibody affinity maturation enables estradiol-17beta assays with more than 10-fold higher sensitivity.
Analytical chemistry - 1 Feb 2010
Kobayashi Norihiro, Oyama Hiroyuki, Kato Yoshinori, Goto Junichi, Söderlind Eskil, Borrebaeck Carl A K
Abstract excerpt
Immunoassays for haptens depend on competitive hapten-anti-hapten reactions, and consequently their sensitivities are significantly influenced by the affinities of anti-hapten antibodies. Thus, genetically engineered antibodies, which have much higher affinities than native antibodies, should increase assay sensitivities. Here, we created a mutated single-chain Fv fragment (scFv) against estradiol-17beta (E(2))...
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