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Article

S-adenosyl methionine prevents promiscuous DNA cleavage by the EcoP1I type III restriction enzyme.

Journal of molecular biology - 17 Oct 2003

Peakman Luke J, Antognozzi Massimo, Bickle Thomas A, Janscak Pavel, Szczelkun Mark D

Abstract excerpt

DNA cleavage by the type III restriction endonuclease EcoP1I was analysed on circular and catenane DNA in a variety of buffers with different salts. In the presence of the cofactor S-adenosyl methionine (AdoMet), and irrespective of buffer, only substrates with two EcoP1I sites in inverted repeat were susceptible to cleavage. Maximal activity was achieved at a Res2Mod2 to site ratio of approximately 1:1 yet...

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PMID: 14529619
DOI: 10.1016/j.jmb.2003.08.042

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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