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Article

Differential stabilization of reaction intermediates: specificity checkpoints for M.EcoRI revealed by transient fluorescence and fluorescence lifetime studies.

Nucleic acids research - 1 May 2008

Youngblood Ben, Bonnist Eleanor, Dryden David T F, Jones Anita C, Reich Norbert O

Abstract excerpt

M.EcoRI, a bacterial sequence-specific S-adenosyl-L-methionine-dependent DNA methyltransferase, relies on a complex conformational mechanism to achieve its remarkable specificity, including DNA bending, base flipping and intercalation into the DNA. Using transient fluorescence and fluorescence lifetime studies with cognate and noncognate DNA, we have characterized several reaction intermediates involving the WT...

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PMID: 18385156
DOI: 10.1093/nar/gkn131
PMCID: PMC2396439

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Source receipt: pubmed, retrieved 7 Sept 2026.

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