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Article

The DNA translocation and ATPase activities of restriction-deficient mutants of Eco KI.

Journal of molecular biology - 1 Oct 1999

Davies G P, Kemp P, Molineux I J, Murray N E

Abstract excerpt

Eco KI, a type I restriction enzyme, specifies DNA methyltransferase, ATPase, endonuclease and DNA translocation activities. One subunit (HsdR) of the oligomeric enzyme contributes to those activities essential for restriction. These activities involve ATP-dependent DNA translocation and DNA cleavage. Mutations that change amino acids within recognisable motifs in HsdR impair restriction. We have used an in vivo...

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Identifiers

PMID: 10525405
DOI: 10.1006/jmbi.1999.3081

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The DNA translocation and ATPase activities of restriction-deficient mutants of Eco KI.PMID 10525405Read ↗

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