Article
Restriction by EcoKI is enhanced by co-operative interactions between target sequences and is dependent on DEAD box motifs.
The EMBO journal - 15 Apr 1996
Webb J L, King G, Ternent D, Titheradge A J, Murray N E
Abstract excerpt
One subunit of both type I and type III restriction and modification enzymes contains motifs characteristic of DEAD box proteins, which implies that these enzymes may be DNA helicases. This subunit is essential for restriction, but not modification. The current model for restriction by both types...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Restriction Enzymes
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Phenotype
- Plasmids
- Sequence Homology, Amino Acid