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Article

Sensitive assay for laboratory evolution of hydroxylases toward aromatic and heterocyclic compounds.

Journal of biomolecular screening - 1 Apr 2005

Wong T S, Wu N, Roccatano D, Zacharias M, Schwaneberg U

Abstract excerpt

Powerful directed evolution methods have been developed for tailoring proteins to our needs in industrial applications. Here, the authors report a medium-throughput assay system designed for screening mutant libraries of oxygenases capable of inserting a hydroxyl group into a C-H bond of aromatic or O-heterocyclic compounds and for exploring the substrate profile of oxygenases. The assay system is based on...

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Source

Identifiers

PMID: 15809320
DOI: 10.1177/1087057104273336

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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