Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A Simple Combinatorial Codon Mutagenesis Method for Targeted Protein Engineering.

ACS synthetic biology - 17 Mar 2017

Belsare Ketaki D, Andorfer Mary C, Cardenas Frida S, Chael Julia R, Park Hyun June, Lewis Jared C

Abstract excerpt

Directed evolution is a powerful tool for optimizing enzymes, and mutagenesis methods that improve enzyme library quality can significantly expedite the evolution process. Here, we report a simple method for targeted combinatorial codon mutagenesis (CCM). To demonstrate the utility of this method for protein engineering, CCM libraries were constructed for cytochrome P450BM3, pfu prolyl oligopeptidase, and the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Codon
Share to Cytochrome P-450 Enzyme System

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Directed Molecular Evolution

Share to Gene Library

Share to Mutagenesis

Share to Mutation

Share to Peptide Hydrolases

Share to Protein Engineering

Source

Open in PubMed Open full text

Identifiers

PMID: 28033708
DOI: 10.1021/acssynbio.6b00297
PMCID: PMC5435451

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A Simple Combinatorial Codon Mutagenesis Method for Targeted Protein Engineering.PMID 28033708Read ↗

SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution.Semantic proximity Read ↗

Codon compression algorithms for saturation mutagenesis.Semantic proximity Read ↗

Mutation Maker, An Open Source Oligo Design Platform for Protein Engineering.Semantic proximity Read ↗

Directed enzyme evolution: beyond the low-hanging fruit.Semantic proximity Read ↗

The diversity challenge in directed protein evolution.Semantic proximity Read ↗

Directed Evolution of Protein Catalysts.Semantic proximity Read ↗

Deep-mutational scanning libraries using Tiled-Region Exchange mutagenesis.Semantic proximity Read ↗

A Brief Guide to the High-Throughput Expression of Directed Evolution Libraries.Semantic proximity Read ↗

Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.Semantic proximity Read ↗

Site-saturation mutagenesis: a powerful tool for structure-based design of combinatorial mutation libraries.Semantic proximity Read ↗

Accelerated protein engineering for chemical biotechnology via homologous recombination.Semantic proximity Read ↗

Expanding the genetic code.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.