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Article

Evolving P450pyr hydroxylase for highly enantioselective hydroxylation at non-activated carbon atom.

Chemical communications (Cambridge, England) - 14 May 2012

Pham Son Quang, Pompidor Guillaume, Liu Ji, Li Xiao-Dan, Li Zhi

Abstract excerpt

Directed evolution of a monooxygenase to achieve very high enantioselectivity for hydroxylation at non-activated carbon atoms is demonstrated for the first time, where a triple mutant of P450pyr hydroxylase is obtained via determination of enzyme structure, iterative saturation mutagenesis, and high-throughput screening with a MS-based ee assay to increase the product ee from 53% to 98% for the hydroxylation of...

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PMID: 22430002
DOI: 10.1039/c2cc30779k

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