Article
Examination and expansion of the substrate range of m-hydroxybenzoate hydroxylase.
Biochemical and biophysical research communications - 20 Jun 2008
Chang Hung-Kuang, Zylstra Gerben J
Abstract excerpt
The gene encoding m-hydroxybenzoate hydroxylase (mobA) was cloned from Comamonas testosteroni GZ39. MobA converts m-hydroxybenzoate and to a lesser extent p-hydroxybenzoate to protocatechuate. To explore the structural and functional relationships in phenolic acid monooxygenases, MobA was subjected to in vitro mutagenesis by error-prone PCR and the mutant MobAs were screened for their ability to oxidize phenol or...
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