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Article

Changing the substrate reactivity of 2-hydroxybiphenyl 3-monooxygenase from Pseudomonas azelaica HBP1 by directed evolution.

The Journal of biological chemistry - 15 Feb 2002

Meyer Andreas, Schmid Andreas, Held Martin, Westphal Adrie H, Rothlisberger Martina, Kohler Hans-Peter E, van Berkel Willem J H, Witholt Bernard

Abstract excerpt

The substrate reactivity of the flavoenzyme 2-hydroxybiphenyl 3-monooxygenase (EC, HbpA) was changed by directed evolution using error-prone PCR. In situ screening of mutant libraries resulted in the identification of proteins with increased activity towards 2-tert-butylphenol and guaiacol (2-methoxyphenol). One enzyme variant contained amino acid substitutions V368A/L417F, which were inserted by two rounds of...

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Identifiers

PMID: 11733527
DOI: 10.1074/jbc.M110018200

Genes

HBP1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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