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Directed Metabolic Pathway Evolution Enables Functional Pterin-Dependent Aromatic-Amino-Acid Hydroxylation in Escherichia coli.

ACS synthetic biology - 20 Mar 2020

Luo Hao, Yang Lei, Kim Se Hyeuk, Wulff Tune, Feist Adam M, Herrgard Markus, Palsson Bernhard Ø

Abstract excerpt

Tetrahydrobiopterin-dependent hydroxylation of aromatic amino acids is the first step in the biosynthesis of many neuroactive compounds in humans. A fundamental challenge in building these pathways in Escherichia coli is the provision of the non-native hydroxylase cofactor, tetrahydrobiopterin. To solve this, we designed a genetic selection that relies on the tyrosine synthesis activity of phenylalanine...

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PMID: 32149495
DOI: 10.1021/acssynbio.9b00488

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